The qPCR and Western blot results showed that when FGFR2 was overexpressed, the expression of biological markers associated with liver fibrosis, including ACTA2 (coding α-SMA), fibroblast activation protein alpha (FAP), and alpha-1 type I collagen (COL1A1), was significantly upregulated in both HSC cells and liver cells (Figure 2A,B). This evidence concerns the gene ACTA1 and Hepatic fibrosis.