Osthole at 100 mg/kg by intraperitoneal route attenuated several clinical and histopathological indicators in the TNBS model of intestinal inflammation in C57BL/6 mice, significantly reducing the IL-1β, TNF-α, IL-6, and COX-2 gene expression and improving intestinal barrier function via upregulation of claudin-1 and zonulin-1 genes [113]. Here, TNF is linked to gastroenteritis.