Therefore, it is found that administration of L. fermentum MG4294 and L. plantarum MG5289 reduced the expression of SREBP1 and FAS related to TG formation and suppressed the expression of PPARγ and C/EBPα, which are adipogenesis-related proteins, through phosphorylation of AMPK in the liver of HFD-induced mice, thereby affecting NAFLD. This evidence concerns the gene PPARG and metabolic dysfunction-associated steatotic liver disease.