As we delved deeper into our perplexing results, we observed that the expression of TFRC and ACSL4 was significantly upregulated in the n-3 PUFA-treated group compared to the PCOS group, while the expression trends of SLC7A11, FTH, and FTL were flipped on their heads (Figure 4f). Here, ACSL4 is linked to polycystic ovary syndrome.