In this work, we systematically investigated the separation performances of columns packed with 300, 420, 500, 620, and 800 nm C18-SiO2 particles for aromatic compounds, PAHs, estrogens, and proteins (lysozyme, cytochrome C, ribonuclease A, and ovarian cancer anti-idiotypic mini body) on a pCEC-UV/Vis platform. The gene discussed is RNASE1; the disease is ovarian carcinoma.