TARDBP and infection: To elucidate the reason why viral particles generated in wt-TDP-43 overexpressing HEK-293T cells lost infection capacity, we precipitated viral particles from synchronous doses of cell-free supernatants (see Materials and Methods) (Figure 3B, see p24 viral control load-associated bands, lines 1–3, quantified in right histograms), and we analyzed Vif and Pr55Gag protein viral content in these viral pellets and normalized p24 content as a control for synchronous viral load (Figure 3B).