GJA1 and CLDN5 were both significantly increased solely in secondary lymphedema, in comparison to both healthy control and lipedema tissue (GJA1: L = 0.999 ± 0.391 fold, LE = 3.132 ± 2.938 fold, H = 1.019 ± 0.596 fold) (CLDN5: L = 1.378 ±0.497 fold, LE = 4.576 ± 3.135 fold, H = 2.856 ± 2.883 fold) and in the case of GJA1, when compared to lipohypertrophy as well). The gene discussed is GJA1; the disease is Lipedema.