Using InForm software analysis, we quantified the intensity of VCAN staining in cancer tissues from five patients in each group and found that the LVI(+) group had a total score of 52.15 compared to 14.13 for the LVI(−) group, indicating that VCAN protein expression was significantly higher in LVI(+) tissues than in LVI(−) tissues (Figure 4C,D). This evidence concerns the gene VCAN and cancer.