CD4 and hepatocellular carcinoma: In particular, by analyzing a cohort of 372 HCC on TCGA database and by using the CIBERSORT algorithm Yang et al. revealed that HCC patients with both high purine biosynthesis and metabolism (PBhiPMhi) are characterized by low levels of M1 macrophages and CD4+ T cells as well as high infiltration of helper T cells and M2 macrophages [204].