TNF and tuberculosis: The percentages of CD4+ and CD8+ effector memory T-lymphocytes (Tem), which produced any combination of IFNγ, IL2, and TNFα in response to in vitro stimulation with the recombinant Ag85A and ESAt-6 proteins, were evaluated by the means of flow cytometry three weeks after a single immunization with TB/FLU-04L (Figure 3).