S100A6 and aortic valve insufficiency: Based on the published correlation between sRAGE level and the appearance of BAV and BAV-associated aortopathies [9], we analyzed the protein expression levels of RAGE and its ligands AGE and S100A6 via Western blot analyses and ELISA, respectively, in the whole-tissue lysates of patients with BAV and TAV (Figure 1) morphology, irrespective of their functional valve status (aortic stenosis/aortic regurgitation).