Thus, by strictly following the conclusions from the cytotoxicity studies on NHA, the potential stimulatory effects on NHA of crude and hot ethanolic extract from fresh and dried Anemone nemorosa at concentration levels lower than 17.6 and 30.8 μg GAE/sample, respectively, these results could suggest AN1 and AN2 potential benefits on brain diseases associated with the decrease of the viability of neuronal cells. The gene discussed is PAX6; the disease is brain disorder.