In order to further validate and strengthen the proteomic findings and based upon our previous demonstration of a consensual higher ubiquitination level associated to the rapid EphB4 degradation in cancer cells [26], we studied the effect of the autocrine IGF-II signal on the specific recruitment of the proteomic-identified factors using a synthetically generated and tagged EphB4 c-tail. The gene discussed is IGF2; the disease is cancer.