RICTOR and pulmonary hypertension, primary, 1: Similarly (Figure 2B,C), RICTOR showed significantly increased phosphorylation (spots 2–5), showing that the incubation of HepG2 cells in CM from PHT cells with mTORC1 inhibition and incubation with CM from PHT cells mTORC2 inhibition resulted in similar patterns of IGFBP-1 hyperphsophorylation.