As shown in Figure 3A–C, CM from PHT cells with mTORC1 inhibition, but not CM from PHT cells with mTORC2 inhibition, markedly increased Ser101, Ser119, and Ser169 phosphorylation of IGFBP-1 secreted from HepG2 cells (Figure 3A–C). This evidence concerns the gene IGFBP1 and pulmonary hypertension, primary, 1.