To explore whether the reduced migrationability of GC cells was associated with EMT, we performed a Western blot analysis to assess the expressionof EMT–related markers, including N-cadherin, E-cadherin, and vimentin in SGC7901.The SHMT2 knockdown resulted in significantly lower levels of N-cadherin and vimentin and higher levels of E-cadherin in GC cells(Figure 3E). This evidence concerns the gene CDH1 and gastric cancer.