Additionally,SHMT2 knockdown induced apoptosis, but the effect was not significant (Figure 2G).We also tested SHIN1, an inhibitor of SHMT2, in the three GC cell lines.The results showed that SHIN1 had a limited ability to kill the cells, with IC50s of 2.31 μM, 2.59 μM, and 2.22 μM,respectively, which were consistent with the knockdown results (Figure S1).We also used SHIN1 for the cell cycle and apoptosis assays (Figures S2 and S3)and the results were consistent with the knockdown results. The gene discussed is SHMT2; the disease is gastric cancer.