This study provides evidence for GR and β-catenin nuclear accumulation in DTX-resistant cells in response to dexamethasone, nuclear GR/β-catenin interaction in mCRPC cells, and re-sensitization of DTX-resistant PCa cells and tumorspheres to DTX by pharmacological co-inhibition of these two proteins. This evidence concerns the gene NR3C1 and posterior cortical atrophy.