Although we observed a mild decrease in the body length of ap1g1+/− (13.62 mm ± 0.0310 mean ± s.e.m.)compared to ap1g1+/+ fish (11.89 mm ± 0.022 mean ± s.e.m.)(Figure 6A,B), there was a significant reduction in the width of the head in ap1g1+/− (anterior head width, 0.0719 mm ± 0.001, and posterior head width, 0.130 mm ± 0.001 mean ± s.e.m.)compared to ap1g1+/+ fish (anterior head width, 0.0780 mm ± 0.001, and posterior head width, 0.135 mm ± 0.001; mean ± s.e.m.)at 6 dpf (Figure 6B), recapitulating microcephaly phenotypes observed in patients. The gene discussed is AP1G1; the disease is microcephaly.