To investigate whether the elevated level of IL-6 played an important role in affecting the survival rate of the 4T1 breast cancer-bearing mice after cryo-thermal therapy, mice bearing 4T1 subcutaneous tumor on Day 16 after implantation were treated with cryo-thermal therapy, and 20 μg of anti-IL-6 mAb or isotype IgG mAb was injected i.p. from Day 7 after cryo-thermal therapy (Figure 2A). The gene discussed is IL6; the disease is breast cancer.