SOD1 and amyotrophic lateral sclerosis: Both pre-treatment and post-treatment with rapamycin suppressed the number of spikes in FUS-ALS and SOD1-ALS motor neurons, although the peak of hyperexcitation overlapped with the timing of administration on day 30, making it difficult to determine the efficacy of treatment with rapamycin in SOD1-ALS neurons (Figure 5B,C).