TNF and basal cell carcinoma: DEGs in the cells in Group 1 (one-time cryopreservation) and in Group 2 (two-time cryopreservation) were mainly enriched in the pathways of parathyroid hormone synthesis, secretion, and action; MAPK signaling pathway; cellular senescence; cell cycle; TNF signaling pathway; basal cell carcinoma; FoxO signaling pathway; and p53 signaling pathway (Figure 1D).