CUMS was used to establish depression and anxiety-like behaviour in rats. The rTMS was performed with a commercially available stimulator for 7 days, and then depression and anxiety-like behav were Measur. Nrf2 expression was Measur by WB and TNF-α, iNOS, IL-1b, IL-6 Measur with ELISA. A small interfering RNA was employed to knockdown Nrf2, after which the neurobehav assessment, Nrf2 nuclear expression, and the amount of inflammation factors were evaluated. Here, NFE2L2 is linked to major depressive disorder.