To test CLA and FO modulation of the Nrf2 pathway in a mouse depression model, brain samples from 2 groups (n = 8 each), composed of 8- (Young) or 22-wk old (Old) mice were examined to evaluate the age-dependent occurrence of depressive disorder markers (BDNF, TrkB, Synaptophysin, Synapsin I; Synaptotagmin I, PPAR-α, PPAR-γ and the modification of DHA, C18:1, C16:0, and C18:0 content) with rtPCR and WB. 2 additional groups composed of 17-wk-old mice (n = 8 each) were supplemented with FO or CLA × 5 wk, when they reached old age, and were compared with untreated Old mice. Here, PPARA is linked to major depressive disorder.