CUMS was used to establish depression and anxiety-like behaviour in rats. The rTMS was performed with a commercially available stimulator for 7 days, and then depression and anxiety-like behav were Measur. Nrf2 expression was Measur by WB and TNF-α, iNOS, IL-1b, IL-6 Measur with ELISA. A small interfering RNA was employed to knockdown Nrf2, after which the neurobehav assessment, Nrf2 nuclear expression, and the amount of inflammation factors were evaluated. The gene discussed is TNF; the disease is major depressive disorder.