Figure 5A shows that CM from preadipocytes pretreated with TNFα significantly stimulated cancer epithelial migration. Similar to the monocyte recruitment, we also analyzed whether MCP1/CCL2 stimulated the MCF-7 cell migration. Our results indicate that the presence of a specific anti-CCL2 blocking antibody (in contact with migratory epithelial cells only during the time of assay) totally inhibits the epithelial migration stimulus of conditioned media from TNFα-pretreated preadipocytes (Figure 5B). Here, CCL2 is linked to cancer.