Since the pro-inflammatory milieu produces epithelial-mesenchymal transition (EMT) and mitochondrial dysfunction in several models [25], we assessed whether the CM derived from TNFα-treated human mammary preadipocytes changed the snail gene expression, an early EMT marker, and mitochondrial ROS (mtROS) levels in MCF-7 cancer epithelial cells. This evidence concerns the gene SNAI1 and cancer.