This hypothesis has already been proven in BRAFV600E-mutated colon cancer by showing that knockdown of RPA1 augments cytotoxic effects of 5-fluorouracil (5-FU) in BRAFV600E-mutated HT-29 colon cancer cells by significantly reducing the proliferative capability of cells measured by the colony formation assay and enhancing 5-FU-induced apoptosis [35]. Here, RPA1 is linked to colonic neoplasm.