Immunoblotting showed that the expression levels of Collagen 1 and TGF-β1 were significantly increased in the TAC group (p < 0.001), but overexpression of PINK1 attenuated the expression of Collagen 1 and TGF-β1 (Figure 2F, File S1). Here, TGFB1 is linked to persistent truncus arteriosus.