The diagnosis of PBC can be established if two of three objective criteria are present: unexplained elevated ALP ≥ 1.5 times the upper normal value for at least 6 months, serum AMA titers ≥ 1:40, or if AMA negative, positivity of anti-sp100/anti-gp 210 and compatible liver histology. This evidence concerns the gene SP100 and primary biliary cholangitis.