We observed that the proportion of immune cells (CD8+ cytotoxic T-cells, CD15+ neutrophils, CD68+ macrophages, FOXP3+ T-reg cells) relative to ERG+ tumor cells directly correlated with the immune clusters (n = 11 immune-hot and n = 5 immune-cold cases) inferred from NanoString transcriptomic profiling (p = 0.0201) (Supplementary Fig. 2). Here, ERG is linked to neoplasm.