In our present study, we aimed to discover the role of luteolin in COPD and to determine whether the effects of luteolin on COPD are associated with the regulation of NOX4‐mediated NF‐κB signaling in vitro and in vivo, with the hope to provide a theoretical basis for the treatment of COPD. This evidence concerns the gene NFKB1 and chronic obstructive pulmonary disease.