We aimed to determine whether receptor tyrosine kinase‐like orphan receptor 2 (ROR2) is involved in the occurrence of acute lung injury (ALI) by an animal study and explore the effect of ROR2 downregulation on lipopolysaccharide (LPS)‐treated human lung carcinoma A549 cells by a cytological study. This evidence concerns the gene ROR2 and acute respiratory distress syndrome.