To initiate the fluorescence‐based CRISPR KO screen, RPE1‐Cas9i‐mt‐mKeima cells were transduced with a lentiviral E3 ligase sgRNA library, targeting 606 Ubiquitin E3 ligases, at a multiplicity of infection (MOI) of 0.2 and a 1,000× library coverage. The gene discussed is PRKN; the disease is infection.