Considering this GWAS hit and the observation that 1) AN and BMI share certain loci (Bulik-Sullivan et al., 2015; Hinney et al., 2017; Zheng et al., 2022), 2) AN mainly affects women (Hübel et al., 2019; Mitchell and Peterson, 2020), and 3) our GWAS-related sex-specific analyses may have been affected by insufficient power due to the limited sample size of the AN GWAS, we performed a mutation screen of CKB’s CDS in 192 female patients with AN (acute or recovered) as well as in 192 children and adolescents with severe obesity and 192 healthy-lean controls (see Table 2). This evidence concerns the gene CKB and obesity due to melanocortin 4 receptor deficiency.