Additionally, Wei and Loeken showed that in the presence of hyperglycemia, DNMT3B activity was increased due to an increase in oxidative stress which is responsible for methylation of CpG island and gene silencing of Pax3 gene during neurulation of embryonic stem cells and in embryos isolated from the hyperglycemic pregnant mice [44]. The gene discussed is DNMT3B; the disease is Hyperglycemia.