Furthermore, the alteration of proliferation-related protein expressions in SW480 cells was determined by Western blotting, and it could be seen that the protein expressions of Cyclin D1 and Cyclin A2 in SW480 and SW620 cell lines were remarkably higher in ESM1-mimic group than those in ESM1-NC group (Figure 4A–4D), indicating that ESM1 overexpression can enhance the proliferation ability of CRC cells. The gene discussed is CCND1; the disease is colorectal carcinoma.