In addition, RT-PCR analysis of CIK cells further showed that GCRV infection induced the expression of HSP90 in a temperature-dependent manner, from which asymptomatic infection of GCRV at 18°C barely induced the expression of HSP90, while the higher temperature of 28°C infections significantly induced its expression (Fig 3L), suggesting that GCRV may exploit the expression HSP90 to facilitate viral infection at 28°C. The gene discussed is HSP90AA1; the disease is viral infectious disease.