To further elucidate the relationship between HIPK1 and CREB in regulating pathological cardiac hypertrophy, we performed function‐rescue experiment in vivo by treating mice with both cTnT‐shHIPK1‐AAV9 and cTnT‐driven CREB expression AAV9 (cTnT‐CREB‐AAV9) compared to those injected with cTnT‐CTL‐AAV9, cTnT‐CREB‐AAV9, or cTnT‐shHIPK1‐AAV9 alone. This evidence concerns the gene HIPK1 and cardiac hypertrophy.