We treated mice via tail vein injections of AAV9 that mediated HIPK1 knockdown driven by a cardiac‐specific troponin promoter (cTnT‐shHIPK1‐AAV9) or control AAV9 vectors (cTnT‐CTL‐AAV9) and subsequently followed 1 week later by TAC surgery to induce pathological cardiac hypertrophy. The gene discussed is HIPK1; the disease is cardiac hypertrophy.