However, the related molecular changes in this mechanism need to be further confirmed in diabetes foot ulcer patients; (3) The wound healing rate in the presence of oe-IRF1 + miR-16-5p antagonist was much lower than that upon oe-NC, indicating that transcription factors other than IRF-1 may regulate the expression of miR-16-5p, the regulatory mechanism of which needs to be further explored in future research. This evidence concerns the gene IRF1 and diabetic foot.