In student’s t test, CD133 expression in the colon cancer tissues was significantly different only in non-cancerous mucosa according to pT factor (p = 0.048) by ANOVA (Fig 3E), while there was no statistical difference with regard to other clinicopathological factors in both tumors and non-cancerous mucosa (S2 Fig). This evidence concerns the gene PROM1 and malignant colon neoplasm.