If the most optimal cut-off value of CD133 expression to discriminate colon cancer tissues from the corresponding non-cancerous mucosa is selected as 1.20 (AUC = 0.62, p = 0.10), high expression of CD133 is again significantly associated with right colon cancer, but not with other clinicopathological factors in primary colon tumors (S2 Table). Here, PROM1 is linked to malignant colon neoplasm.