We investigated the effect of three generations of mTOR kinase inhibitors alone and in combination with the MEK1/2 kinase inhibitor AS-703026 on the expression of pro-survival proteins: p-Bcl-2 (T56), Bcl-2, Bcl-xL, and Mcl-1 in MEWO melanoma cell line. The gene discussed is BCL2L1; the disease is melanoma.