To further assess the effect of IFIT1 and RSAD2 knockout on BTV replication, BovFibT, IFIT-KO cells, and RSAD2-KO cells were either stimulated with uIFN or mock treated for 24 h prior to infection with wild-type BTV-8 (multiplicity of infection [MOI] of 0.01) (Fig. 3E). This evidence concerns the gene IFIT1 and infection.