RAB27A and hepatocellular carcinoma: HCC animal model with bone lesions was established by inoculating LM3 cells into the liver, followed by intratibial injection of phosphate buffered saline (PBS), negative control cells (LM3/Lck‐GFP sh‐NC or BM4/Lck‐GFP sh‐NC), or BM4/Lck‐GFP cells with RAB27A knockdown (sh‐Rab27a), which suppressed EV secretion (Figure 1D and Figure S1C,D, Supporting Information).[25] Gross appearance and H&E staining of liver and bone, as well as bioluminescence imaging (BLI) and GFP signals in bone, confirmed the success of the animal model (Figure 1E,F and Figure S1E–I, Supporting Information).