The most common gene alterations for the 301 CRC patients were APC (77%), TP53 (73%), KRAS (48%), PIK3CA (25%), FBXW7 (22%), SMAD4 (18%), TCF7L2 (17%), LRP1B (15%), FAT4 (14%), ARID1A (13%), ACVR2A (13%), SOX9 (13%), RNF43 (11%), PIK3R1 (10%), and SPTA1 (10%) (Figure 1A). Here, SMAD4 is linked to colorectal carcinoma.