The results showed that the AUC of AFP and EV-DLEU2 in mUICC stage I HCC were 0.508 and 0.885, respectively, compared with those in NL, CH, and liver cirrhosis, indicating that EV-DLEU2 performed significantly better than AFP in the detection of HCC at the very early stage. The gene discussed is AFP; the disease is hepatocellular carcinoma.