To this end, cells were grouped into five major PBMC cell types: ‘B cells’ (B-memory + naïve B cells), ‘CD4+ T cells’ (naïve and memory), ‘CD8+ T cells’ (naïve and memory), ‘myeloid cells’ (classical monocytes, non-classical monocytes, pDCs and mDCs) and ‘other’ cell types (NK cells and double-negative T cells) and differential accessibility analysis performed with DESeq2 in a pseudo-bulk manner, comparing 4 HC samples to 4 ALS samples for every of the major cell types as recommended before to avoid pseudo-replication [39]. This evidence concerns the gene CD8A and amyotrophic lateral sclerosis.