We confirmed that the phosphorylation levels of STAT3 in primary tumor tissues from mice injected with wild-type TMEM25-encoded AAV were significantly lower than those from mice injected with control AAV, or TMEM25-R326W or TMEM25-L338F encoded AAV by immunoblotting assay (Supplementary Fig. 10c). This evidence concerns the gene STAT3 and neoplasm.