Here, the CFTR locus of affected organoids was modified by insertion of a normal CFTR gene using CRISPR/Cas9, with targeted organoids displaying a restored swelling response to forskolin induced swelling assay.56 In colorectal cancer, CRISPR/Cas9 is exploited to develop a closer human colorectal cancer model using HIOs. The gene discussed is CFTR; the disease is colorectal cancer.