The main differences between the 30‐week‐old KO and A537T mice independent of the TRAMP background were predominately the activation of transport and RNA processing pathways in KO/KO‐TRAMP mice and an upregulation in cell cycle and cell growth processes via WNT signalling and IGF transport and uptake in A537T/A537‐TRAMP mice, reflective of prostate tumorigenesis and cancer progression compared with prostate enlargement in the KO and A537T mice (Appendix Fig S7B and D). Here, IGF1 is linked to male reproductive organ cancer.