The tumor-to-blood and tumor-to-muscle ratios of [68Ga]Ga-NOTA-anti-CD70 VHH in CD70high tumors were 9.99 ± 2.83 and 18.93 ± 7.85, respectively, and were significantly higher than those in CD70low tumors (T/B: 1.84 ± 0.06; p = 0.0076, and T/M: 3.02 ± 1.10; p = 0.0254). Here, CD70 is linked to neoplasm.