To validate the role of STK11/LKB1 in regulating host wasting, the H1792 NSCLC cell line was infected with lentiviral CRISPR/Cas9 constructs possessing guide RNA directed against the kinase domain of STK11/LKB1 (H1792ΔSTK11) to silence expression of the gene as described in Methods. The gene discussed is STK11; the disease is non-small cell lung carcinoma.