CD45+Ly6G–SSChiCD11c+Lyz2-YFPhi AMs were isolated by flow sorting from single-cell suspensions of non–tumor-bearing whole lungs collected from Lyz2-Cre+/+YFP+/–Ctnnb1Ex3Δ/wt or Lyz2-Cre+/+YFP+/– control mice in biologic triplicates (Supplemental Figure 4A). The gene discussed is PTPRC; the disease is neoplasm.