To confirm that the interaction between 14-3-3 and the fragment of PABPC1 detected in the screen reflected an interaction between 14-3-3 and the full length PABPC1 molecule in cells, total cell lysates from the breast cancer cell line MCF7 were incubated with either GST alone or GST-14-3-3β/ζ fusion proteins in a pulldown assay and probed for PABPC1 by Western blotting. The gene discussed is PABPC1; the disease is breast cancer.