In order to assess the capability of SIEVE for the analysis of these more complex systems, we engineered the murine pancreatic cancer cell line K8484 (27) derived from the KPC (Trp53R172H, KrasG12D, Pdx1-Cre) mouse model (28) to express the SORT machinery and mCherry-H2B (K8484_SORTGCU). Here, H2BC21 is linked to familial pancreatic carcinoma.