To assess specificity of SIEVE compared with generic EV isolation with PS-capture in this fibroblast and cancer cell coculture, we compared triplicates of PS (Tim4)- and SIEVE (SPIEDAC)-purified EV samples of four conditions consisting of both monocultures, the K8484_SORTGCU and L cells coculture, as well as mixed monoculture samples admixing EVs from each cell lines in a 1:4 ratio to match the seeding ratio of the coculture. This evidence concerns the gene TIMD4 and cancer.